Transcriptomics of L. monocytogenes treated with olive leaf extract

L. monocytogenes F2365 strain was grown in Brain Heart Infusion (BHI) liquid broth overnight at 30°C with 200 rpm agitation. The stationary phased bacterial cells were diluted 1:50 in BHI liquid broth supplemented with 7.8 mg/ml olive leaf extract, grown at 30°C with 200 rpm agitation for 3.5 h and 24 h, respectively. The bacterial cells without olive leaf extract grown at the same conditions (3.5 h and 24 h) were used as controls. Bacterial RNA was isolated using RiboPure RNA Purification Kit (Thermo Fisher Scientific). rRNA was depleted from total RNA using Ribo-Zero Magnetic Kit (Illumina). For fragmentation and library preparation, Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) was used according to the manufacturer’s instruction. RNA and DNA quality and library quantification were performed using using a 2100 Bioanalyzer instrument (Agilent Technologies). Sequencing was carried out using Ion Chef and Ion S5 instruments (Thermo Fisher Scientific).