Mitochondrial function in PBMCs of healthy donors and ME/CFS patients (CTRL + wIRA)

Our research hypothesis was that heat treatment by water-filtered infrared-A irradiation (wIRA) at 39 °C for 60 minutes would lead to alterations in mitochondrial function compared to an untreated control. PBMCs from healthy donors and ME/CFS patients were studied. The following files show the mitochondrial parameters of ATP production, basal respiration, maximal response, spare respiratory capacity and non-mitochondrial respiration as oxygen consumption rate (OCR) per 1000 cells. Data are presented as mean values calculated from a minimum of two and a maximum of eight replicates per condition. The data show a higher mitochondrial function of the measured parameters in ME/CFS patients compared to healthy donors. Heat treatment compensates for these higher values and leads to an approximation to the healthy level.

本研究之假设为，经水过滤红外-A (wIRA) 照射处理的热处理，于39°C下持续60分钟，相较于未经处理的对照组，将导致线粒体功能的改变。本研究以健康捐献者和慢性疲劳综合症/纤维肌痛（ME/CFS）患者的PBMCs为研究对象。以下文件展示了线粒体参数，包括ATP产生、基础呼吸、最大反应、储备呼吸能力和非线粒体呼吸（以每1000个细胞的氧消耗率OCR表示）。数据以每条件至少两次、最多八次的重复实验的平均值呈现。数据显示，与健康捐献者相比，ME/CFS患者的测量参数线粒体功能更高。热处理补偿了这些较高值，并使线粒体功能接近健康水平。