Supporting data for “Deregulation of RNA acetylation and NAT10 in liver cancer: from molecular mechanism to therapeutics”

Epitranscriptomics is a burgeoning research area that concerning various modifications on RNA. N6-Methyladenosine (m6A) was the most famous and the best characterized modification on eukaryotic mRNA with the known function to affect mRNA splicing, translation, and degradation. While N1-Methylpseudouridine (m1Ψ) was another crucial one that solved the bottlenecks for in vivo mRNA delivery so that made mRNA vaccine possible in the past COVID-19 pandemic. However, the functions of other mRNA modifications, especially for their roles in human physiology and pathology remain largely unknown. Herein, we revealed the crucial oncogenic functions of N4-acetylcytidine (ac4C) and RNA cytidine acetyltransferase NAT10 in facilitating human liver carcinogenesis.Our work also demonstrated that, RNA cytidine acetyltransferase NAT10 could be a novel and promising therapeutic target for advanced liver cancer treatment.

表观转录组学是一个新兴的研究领域，专注于RNA的各种修饰。N6-甲基腺苷（m6A）是其最著名且表征最清晰的修饰之一，已知其功能可影响mRNA的剪接、翻译和降解。而N1-甲基伪尿苷（m1Ψ）则是另一个关键的修饰，它解决了体内mRNA递送的限制，使得在过去的COVID-19大流行期间mRNA疫苗成为可能。然而，其他mRNA修饰的功能，尤其是在人类生理学和病理学中的作用，仍知之甚少。本研究揭示了N4-乙酰胞苷（ac4C）和RNA胞苷乙酰转移酶NAT10在促进人类肝细胞癌发生中的关键致癌作用。我们的研究还表明，RNA胞苷乙酰转移酶NAT10可能成为治疗晚期肝癌的一种新颖且具有潜力的治疗靶点。