Plant-mediated interactions between Tetranychus evansi and T. urticae spider mites - suppression test 3.

We infested all leaflets of the 3^rd leaf of 28-days old tomato plants (<i>Solanum lycopersicum</i> var Santa Clara) with spider mites in three treatments: (1) 20 two to four days old adult <i>Tetranychus evansi</i> Viçosa-1 females, (2) 10 two to four days old adult <i>Tetranychus urticae</i> Santpoort-2 females, or (3) no infestation. These treatments lasted two days, after which we removed mites, eggs and web, and cut up to 10 leaf discs per plant from the previously infested leaflets. Then, we placed two to four days old adult <i>Tetranychus evansi</i> Viçosa-1 females individually on each leaf disc, and counted the number of eggs of each leaf disc after two and five days. These experiments were carried out in a greenhouse.

我们对28日龄番茄植株（<i>Solanum lycopersicum</i> 圣克拉拉品种）第3片叶的所有小叶开展三种螨类接种处理：（1）接入20头2~4日龄的成年<i>Tetranychus evansi</i> Viçosa-1雌性成螨，（2）接入10头2~4日龄的成年<i>Tetranychus urticae</i> Santpoort-2雌性成螨，（3）不进行螨类接种。上述处理持续2天后，我们清除所有螨体、卵粒与丝网，并从此前接种过螨类的小叶上，每株植株切取至多10张叶碟。随后，将2~4日龄的成年<i>Tetranychus evansi</i> Viçosa-1雌性成螨单独放置于每张叶碟上，并分别于接种后2天和5天，统计每张叶碟上的产卵量。本实验于温室中开展。