Spatial Multimodal analysis (SMA) - Mass Spectrometry Imaging (MSI)

The files above are MALDI-MSI (Matrix Assisted Laser Desorption Ionization - Mass Spectrometry Imaging) experiments. The dataset contains both imzML, which is the common MSI open file type, and csv, containing x, y positions in tissue as well as m/z and intensity, which is a more user friendly format. A major difference between the two types is that the imzML contains raw data, whereas the csv data is peak-picked, explaining the discrepancy in file sizes. The data contained in this dataset should be enough to recreate the MSI parts of the manuscript.
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The purpose of the experiments was to demonstrate that MALDI-MSI and ST (Spatial Transcriptomics) could be done on the same tissue. MALDI-MSI was done first, and ST second, so there is no expected difference in the tissue compared to a regular MALDI-MSI run, however, the experiments where ST was run subsequent to MALDI-MSI required non-conductive glass which is not the standard in the MSI- field. To compare, experiments done on the commonly used conductive (ITO) glass slides are also contained in this dataset
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For any questions about the dataset or to request access to any files not uploaded here, please contact [e-mail]

上述文件均属于基质辅助激光解吸电离-质谱成像（Matrix Assisted Laser Desorption Ionization - Mass Spectrometry Imaging，MALDI-MSI）实验数据集。本数据集同时包含两种格式文件：其一为当前质谱成像领域通用的开源文件类型imzML；其二为更便于用户使用的csv格式文件，其中存储了组织内的x、y坐标位置以及质荷比（m/z）与信号强度信息。两种格式的核心差异在于：imzML存储原始实验数据，而csv格式则经过了峰拾取处理，这也是二者文件大小存在差异的原因。本数据集包含的数据足以复现研究手稿中质谱成像部分的相关实验结果。
本实验的核心目的为验证：可在同一块组织切片上先后开展MALDI-MSI与空间转录组学（Spatial Transcriptomics，ST）实验。实验顺序为先进行MALDI-MSI，再开展空间转录组学实验，因此相较于常规MALDI-MSI实验，该处理后的组织样本理论上无显著差异。但需注意，先完成MALDI-MSI再开展空间转录组学的实验流程，需要使用非导电玻璃载玻片——这并非质谱成像领域的标准载玻片类型。为便于对比分析，本数据集同时收录了使用质谱成像领域常用的导电（ITO）玻璃载玻片开展的实验数据。
若您对本数据集有任何疑问，或申请获取未在此处上传的文件，请联系邮箱[e-mail]。