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The Fidelity of DNA Polymerase in Microgravity

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA773952
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DNA polymerase fidelity and replication rates were assayed under conditions of microgravity generated by parabolic flight and compared to earth-like gravity. Upon commencement of a parabolic arc, primed synthetic single-stranded DNA was used as a template for one of two enzymes (Klenow fragment exonuclease+/-; with and without proofreading exonuclease activity, respectively in triplicate) and were quenched immediately following the 20 second microgravitational period.
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2021-10-23
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