Transcriptome analysis of HeLa cells knocked down for EXT1 or FBXW7 gene expression

Exostosin-1 (EXT1) is an ER-resident glycosyltransferase known to polymerize heparan sulfate chain by sequential addition of glucuronic acid and N-acetylglucosamine molecules. This reaction results in heavily glycosylated proteoglycans (PG) at the plasma membrane, which form cell-type specific complexes and play vital roles in cell-cell and cell-pathogens interactions, cell growth, plasticity and proliferation in the local tissue environment. We found that EXT1 interact with a number of intracellular proteins including FBXW7 and hypothesized that both proteins cooperate in targeted protein degradation. Overall design: Examination of transcriptomic differences following knockdown of EXT1 or FBXW7.