Identification of Eda targets during Meibomian gland development

Eda signaling plays critical role for Meibomian gland dvelopment, however, the crosstalk of Eda with other signaling is largly unknown. By comparing expreession profilings between wild-type and Eda mutant Tabby eyelids, we identified Dkk4 and Lrp6 highly expressed during mouse Meibomian gland development. Total RNAs isolated from microdissected wild-type and Tabby eyelids were profiled with Agilent 44K NIA mouse microarrays.Eda信号通路（Eda signaling）在睑板腺（Meibomian gland）发育过程中发挥关键调控作用，然而目前Eda与其他信号通路的串扰机制仍鲜为人知。本研究通过对比野生型（wild-type）与Eda突变型Tabby小鼠眼睑的基因表达谱，鉴定出在小鼠睑板腺发育阶段高表达的Dkk4与Lrp6基因。研究人员从显微切割获取的野生型及Tabby小鼠眼睑组织中分离总RNA，并采用Agilent 44K NIA小鼠基因芯片完成表达谱分析。