Malus fusca Transcriptome or Gene expression

The objective of this project is to generate transcriptional sequence data from a variety of structures from Malus fusca (Oregon Crab Apple), grown under field conditions or in controlled environment, at multiple time points over the course of one growing season and during development. The data can be linked with ChIP-seq data targeting transcriptionally relevant modifications of histone H3. Results may be used to facilitate sequence analysis of gene-rich regions in plants with complex genomes, identify chromatin landmarks useful for genome annotation, and identify polymorphic sequences most useful for genetic map construction. The project is expected to help uncover how chromatin structure is linked to gene activity, add accuracy to annotation efforts, and uncover cryptic and previously unanticipated features of plant genomes.