Stimulation of canine joint cells to model osteoarthritis

Canine chondrocytes and osteoblasts were embedded in an agarose matrix and simultaneously stimulated with cytokines whilst being cyclically compressed. Both cell types were compressed for 4 hours at 1 Hz followed by a 24 hour recovery incubation. The chondrocytes were stimulated with 5 ng/mL of IL8 for the duration of compression, but removed for the recovery phase. Osteoblasts were stimulated with 0.5 ng/mL IL8 and 0.5 ng/mL IL1b for the duration of the compression and for the entirety of the 24 hour incubation step.

将犬软骨细胞（chondrocytes）与成骨细胞（osteoblasts）包埋于琼脂糖基质（agarose matrix）中，在施加周期性压缩载荷的同时以细胞因子（cytokines）进行刺激。两种细胞均以1 Hz频率施加压缩载荷持续4小时，随后进行24小时的恢复培养。软骨细胞仅在压缩载荷施加阶段以5 ng/mL的白细胞介素8（IL8）进行刺激，恢复阶段则停止刺激。成骨细胞则在整个压缩过程以及24小时培养的全程中，均以0.5 ng/mL的白细胞介素8（IL8）与0.5 ng/mL的白细胞介素1β（IL1β）开展联合刺激。