Is the colon mucosa affected by ten days of gastric restriction in an animal model?

Abstract Purpose To identify whether the colon mucosa is affected by ten days of gastric restriction in an animal model. Methods An experimental model of gastric restriction was devised using rats. The animals were submitted to surgical gastrostomy, and a cylindrical loofah was inserted into the stomach. We studied 30 adult male Wistar rats divided into three groups: the stomach restriction group (R10); the sham group (S10), which underwent the same procedure except for the loofah insertion; and the control group (C10). The expression of neutral and acid mucins was evaluated using histochemical techniques. Goblet cells and protein content were compared between groups using generalized estimation equations (GEEs). Bonferroni’s multiple comparison was applied to identify differences between the groups. All tests considered a 5% significance level. Results There was an increased expression of neutral mucins, acid mucins and goblet cells in the R10 group. Collagen was also enhanced in the R10 group. Conclusion The colon mucosa is affected by ten days of gastric restriction in an animal model, increasing neutral mucins, acid mucins and collagen content with trophic maintenance.

摘要
目的：明确动物模型中，为期10天的胃限饲是否会对结肠黏膜造成影响。
方法：本研究以大鼠为实验对象构建胃限饲实验模型。对实验动物实施外科胃造瘘术，并向胃内植入圆柱形丝瓜络。本研究共纳入30只成年雄性Wistar大鼠，分为三组：胃限饲组（R10）、假手术组（S10，仅未植入丝瓜络，其余操作与胃限饲组一致）以及对照组（C10）。采用组织化学技术检测中性黏蛋白与酸性黏蛋白的表达水平；通过广义估计方程（generalized estimation equations, GEEs）比较各组的杯状细胞数量与蛋白质含量，并采用Bonferroni多重比较检验分析组间差异，所有检验均以5%作为显著性水准。
结果：胃限饲组（R10）的中性黏蛋白、酸性黏蛋白及杯状细胞表达水平均有所升高，该组的胶原含量同样得到提升。
结论：在动物模型中，为期10天的胃限饲会对结肠黏膜产生影响，可提升中性黏蛋白、酸性黏蛋白与胶原的含量，并维持组织营养状态。