Supplementary Videos: Whole-Mount mesoSPIM Imaging of INSIHGT-BABB-Cleared Wildtype E7 Chicken Embryos Immunolabelled for Islet-1 (Video 1) and Neurofilament (Video 2)

This dataset contains two supplementary video animations of whole-mount immunolabelled, optically cleared wildtype E7 chicken embryos, acquired as part of a Master's thesis investigating the impact of 1-deoxy-sphingolipids on neurodegeneration in vivo (ETH Zurich / University Hospital Zurich). Embryos were immunolabelled using the INSIHGT (In Situ Host-Guest Chemistry for Three-Dimensional Histology) whole-mount staining protocol and optically cleared using the BABB (benzyl alcohol/benzyl benzoate) method. Whole embryos were imaged in their entirety using a mesoSPIM benchtop light-sheet microscope. Individual image tiles were stitched using the BigStitcher plugin for Fiji/ImageJ, and the resulting volumetric datasets were rendered into video animations using Imaris. Video 1 — Islet-1: Whole-mount Islet-1 immunolabelling (embryo LW290), imaged at 7.5× magnification (xy-resolution 0.56 µm, z-step 10 µm). Resolves individual Islet-1-positive sensory neuron nuclei within the DRGs and Islet-1-positive motor neuron nuclei within the spinal cord. This video is displayed at 18-fold reduced resolution relative to the native mesoSPIM acquisition (32-bit to 16-bit conversion and 3× downsizing in x/y) Video 2— Neurofilament (NF): Whole-mount NF immunolabelling (embryo LW73), imaged at 5× magnification (xy-resolution 0.85 µm, z-step 10 µm). Resolves the peripheral nervous system across the full length of the embryo, including segmentally repeating dorsal root ganglia (DRGs) and their peripheral nerve branches. This video is displayed at 8-fold reduced resolution relative to the native mesoSPIM acquisition (32-bit to 16-bit conversion and 2× downsizing in x/y) Together, these videos represent the first three-dimensional whole-mount visualization of the DRG achieved in this chicken embryo model, demonstrating homogeneous antibody penetration from the tissue surface to the level of individual cell nuclei.