Light-sensitive Ca2+ signaling in the mammalian choroid

The choroid is the thin, vasculature-filled layer of the eye situated between the sclera and the retina, where it serves the metabolic needs of the light-sensing photoreceptors in the retina. Illumination of the interior surface of the back of the eye (fundus) is a critical regulator of subretinal fluid homeostasis, which determines the overall shape of the eye, but it is also important for choroidal perfusion. Noted for having some of the highest blood flow rates in the body, the choroidal vasculature has been reported to lack intrinsic, intravascular pressure-induced (myogenic) autoregulatory mechanisms. Here, we ask how light directly regulates choroid perfusion and ocular fluid homeostasis, testing the hypothesis that light facilitates ocular fluid absorption by directly increasing choroid endothelial permeability and decreasing choroid perfusion. Utilizing ex vivo pressurized whole-choroid and whole-eye preparations from mice expressing cell-specific Ca2+ indicators, we found that ..., High-speed, high resolution spinning disk confocal/widefield imaging obtained from ex vivo pressurized and unpressurized choroid preparations.  Images in TIFF format will be generated using commercial software (VisiView), but images and metadata are freely accessible using open-source software such as ImageJ. Immunofluorescence/brightfield microscopy images generated from pinned-down en face retinal vasculatures. Raw z-stack images will be saved in either TIFF or AVI format and can be freely transformed within open-source software., , # Data from: Light-sensitive Ca2+ signaling in the mammalian choroid

[https://doi.org/10.5061/dryad.h70rxwdtn](https://doi.org/10.5061/dryad.h70rxwdtn)

## Description of the data and file structure

This dataset contains all the processed data published in \"Light Sensitive Ca2+ Signaling in the Mammalian Choroid\".

**Figure1A_processed_data.xlsx:** This file contains averaged calcium fluorescence traces from choriocapillaris endothelial cells expressing GCaMP6f during 405 nm light stimulation. Imaging was performed using 470 nm excitation at 5 Hz.

***Figure1_A1_Data tab:***

Column A: Time (s): Time in seconds. A value of 0 s indicates the onset of 405 nm light stimulation.

Column B: High Power Cell 1: Raw fluorescence intensity values for Cell 1 during high-power stimulation.

Column C: High Power Cell 2: Raw fluorescence intensity values for Cell 2 during high-power stimulation.

Column D: High Power Cell 3: Raw fluorescence intensity values for Cell 3 during high-power stimulatio..., ,