Mouse glioblastoma single cell RNA sequencing (.rds file)

GL261 cells (1E4 for each injection) in DMEM medium were injected intracranially with a stereotactic apparatus (Kopf Instruments) at coordinates 2.2 mm lateral relative to Bregma point and 2.5 mm deep from dura mater and GBM samples were collected at 22 days post-injection for single cell RNA seq.

将DMEM培养基中的GL261细胞（每注射剂量为1×10⁴个细胞）通过立体定位仪（Kopf Instruments）进行颅内注射，注射坐标为相对于前囟点（Bregma）外侧2.2mm、距硬脑膜深度2.5mm；于注射后22天收集胶质母细胞瘤（GBM）样本，用于单细胞RNA测序（single cell RNA seq）。