qPCR of plant, fungal, and endobacterial genes from RNA.

Values indicate the mean (n = 2) qPCR cycle number at which amplification reached the threshold of detection (Ct) for each locus tested in each cDNA library from the Arabidopsis thaliana root RNA samples used in the RNAseq experiment. Dash = not tested. Arabidopsis GADPH and Linnemannia elongata RPB1 are single copy genes. The bacterial 16S gene is multicopy, which was necessary for detection, since these endobacteria are in very low abundance in the fungal hyphae. (XLSX)