Changes in gene expression of H. pylori 26695 and ?dppA cells

To further examine the roles of DppA, we performed RNA-seq analysis and investigated the genes expressed differentially between H. pylori 26695 and a ?dppA strain.We found that 253 genes were differentially expressed with a |Log2 (fold change)| > 1, including 116 genes that were upregulated and 137 genes that were downregulated in ?dppA (P < 0.05). We also performed functional classification of the genes upregulated and downregulated in ?dppA. We found that those genes involved in energy metabolism, transportation, and translation were significantly downregulated in ?dppA, which might contribute to the decreased growth rate of H. pylori in this genetic background. Genes involved in DNA metabolism, bacterial pathogenesis, and motility were upregulated in this strain, and they might contribute to the virulence of H. pylori. Overall design: To prepare total RNA for transcriptomic study, H. pylori 26695 and ?dppA cells were cultivated in Brucella broth containing 10% FBS for 20 h until exponential phase in a shaker at 120 rpm in a microaerobic environment (5% O2, 10% CO2, and 85% N2) at 37°C.Two replicates was done per sample