RNA-Seq analysis of knocking out Fndc5 on ischemia-reperfusion injury induced transcriptome changes in mouse skeletal muscle. Mus musculus

Ischemia/Reperfusion (I/R) injury is caused by the restoration of blood supply to ischemic tissues, which exacerbates the damage to the ischemic tissue. Skeletal muscle I/R injury is a common pathology that may occur in severe osteo-soft tissue injury, amputation reimplantation, osteo-fascial compartment syndrome, crush syndrome, vascular injury and chronic arterial occlusive disease. FNDC5/Irisin is an identified muscle factor that converts white adipose tissue to brown adipose tissue.Several studies have shown that irisin is associated with ischemia-reperfusion injury in organs, including the brain, lungs, heart, liver, kidneys, and intestines. Although studies of exogenous irisin ameliorating I/R injury in skeletal muscle have been reported, the mechanism of action is unclear. Here, to better understand how FNDC5 alters skeletal muscle function and affects skeletal muscle ischemia-reperfusion injury, we performed RNA-seq analysis of wild-type (WT) and FNDC5 knockout mice based on RNA-seq technology that knocked out the FNDC5 gene to produce mice lacking irisin.