Deetect: a deep learning based image analysis tool for quantification of adherent cell populations on oxygenator membranes after extracorporeal membrane oxygenation therapy

A phosphorylcholine-coated (PC) polymethylpentene (PMP)-MOs (Maquet PLS-I) was examined for cell colonization after long-term patient support (>24h) using confocal laser scanning microscopy (Leica SP5/40X) and fluorescence staining of adhering cells via 4',6-Diamidino-2-phenylindol (DAPI). The cell colonization was found to be localized at certain predilection sites: hollow fibers and hollow-fiber connecting warps.

采用磷酰胆碱涂层（PC）的多甲基戊烯（PMP）-MOs（Maquet PLS-I）在长期患者支持（>24小时）后，经共聚焦激光扫描显微镜（Leica SP5/40X）和4',6-二氨基-2-苯基吲哚（DAPI）荧光染色对粘附细胞进行染色，对其细胞定植情况进行了研究。研究结果显示，细胞定植呈现特定偏好区域：中空纤维及其连接的纬线。