Metagenome-assembled genomes from Wind River Basin floodplain sediments Riverton, Wyoming site (May to September 2017)

Microorganisms play a key role in cycling nutrients and contaminants in the terrestrial environment depending on their genetic potential. Here we present metagenome-assembled genomes (MAGs) for the bacterial and archaeal community in floodplain sediment samples taken roughly every month in the period May 18 to September 13 in 2017 at a location (Pit2) close to DOE Legacy Management well 855 at the Riverton, Wyoming floodplain site in the Wind River Basin (WRB). The groundwater at this site exhibits persistent U, Mo, and sulfate plumes and is one of the field sites in focus for the SLAC Groundwater Quality SFA program. Cores were taken with a hand-auger and separated into 5-20 cm segments based on soil horizonation down to 150 cm depth below surface. Each segment was subsampled for microbial analyses. Corresponding 16S rRNA gene amplicon data is available at the NCBI Single Read Archive (SRA) Database BioProject ID PRJNA626616, and soil geochemistry data at doi:10.15485/1631972. 40 metagenomes were sequenced through JGI and can be found under Gold sequencing project: Gs0142591. Metagenomes were assembled, binned, and refined using metawrap to generate MAGs (>50% complete and < 10% contamination based on checkM scores). This dataset includes a zip file of 6993 MAG fasta files and a csv file with quality, taxonomic classification (GTDB RS220), and metagenome accessions for MAGs generated from the Wind River Basin (WRB). This dataset also includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type.

微生物凭借自身遗传潜力，在陆地生态系统的养分与污染物循环中发挥关键作用。本研究针对2017年5月18日至9月13日期间，在风河盆地（Wind River Basin, WRB）怀俄明州里弗顿泛滥平原场址、靠近美国能源部（Department of Energy, DOE）遗留管理井855的Pit2点位，按月定期采集的泛滥平原沉积物样品中的细菌与古菌群落，构建了宏基因组组装基因组（metagenome-assembled genomes, MAGs）。
该场址地下水存在持续的铀（U）、钼（Mo）与硫酸盐羽流，是SLAC地下水质量科学焦点区域（SFA）计划重点关注的野外场址之一。
研究采用手持螺旋钻采集岩心，依据土壤发生层将其划分为地表以下0至150 cm深度范围内的5~20 cm层级片段，每个片段均分取子样本用于微生物组分析。
对应的16S rRNA基因扩增子数据可在美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）序列读取存档（Sequence Read Archive, SRA）数据库的BioProject编号PRJNA626616下获取；土壤地球化学数据可通过DOI：10.15485/1631972获取。
本研究共通过联合基因组研究所（Joint Genome Institute, JGI）完成40个宏基因组的测序，相关数据可在Gold测序项目Gs0142591下获取。
宏基因组通过MetaWRAP工具完成组装、分箱与优化，最终得到符合CheckM评分标准的MAGs（完整度＞50%且污染度＜10%）。
本数据集包含6993个MAG的FASTA格式文件压缩包，以及一份涵盖WRB来源MAG的质量评估参数、分类学注释（基于GTDB RS220）与宏基因组登录号的CSV文件。
本数据集还包含文件级元数据文件flmd.csv，用于列出数据集中所有文件及其关联元数据；以及数据字典文件dd.csv，其中收录了所有文件中使用的行列表头，并附带其定义、单位与数据类型。