Optimization of Hydrolases Production (Dataset).pdf

The saccharification of biomass with hydrolases from fungi is considered as an efficient and sustainable approach to obtain fermentable sugars. The expression of these hydrolytic enzymes by fungi is mainly by a substrate-induced mechanism, which depends on prevailing environmental conditions. Trametes polyzona, a white rot fungus, is regarded as one of the most efficient producers of hydrolases for the hydrolysis of biomass. This study sought to examine and optimize cultivation conditions for the efficient production of hydrolases by Trametes polyzona (T. polyzona). Hydrolase production by T. polyzona was examined using parametric optimization. Crude enzyme extracts obtained under varying cultivation conditions were profiled for endoglucanase, betaglucosidase, exoglucanase, xylanase and amylase activities to determine the optimal conditions for hydrolase production. Maximum hydrolase production was achieved by solid-state cultivation of T. polyzona on cassava peels at a moisture content of 60%, pH 6, supplemented with 0.3% urea and 0.03% Mg2+, and incubated at 30 oC for 14 days. A solid to liquid ratio of 1:2 was found to be ideal for the solid-liquid extraction of enzymes. This study therefore proves that optimization of cultivation conditions is an efficient approach to enhance the production of hydrolases required to breakdown lignocellulosic biomass by T. polyzona.

利用真菌源水解酶（hydrolase）进行生物质糖化，是获取可发酵糖的高效且可持续的途径。真菌对这类水解酶的表达主要通过底物诱导机制实现，且该过程受当前环境条件的影响。多带栓菌（Trametes polyzona）作为一种白腐真菌（white rot fungus），被视为用于生物质水解的高效水解酶生产菌株之一。本研究旨在探究并优化多带栓菌（T. polyzona）高效生产水解酶的培养条件。本研究采用参数优化法考察多带栓菌的水解酶生产情况，针对不同培养条件下获取的粗酶提取物，开展内切葡聚糖酶（endoglucanase）、β-葡萄糖苷酶（beta-glucosidase）、外切葡聚糖酶（exoglucanase）、木聚糖酶（xylanase）与淀粉酶（amylase）的活性谱分析，以确定水解酶生产的最优条件。当以木薯皮（cassava peels）为底物，采用固态发酵（solid-state cultivation）方式，在含水量60%、pH值6、添加0.3%尿素与0.03%镁离子（Mg²+）、30℃下培养14天时，多带栓菌的水解酶产量达到峰值。研究证实，固液比1:2为酶的固液提取的最优比例。综上，本研究证实，优化培养条件是提升多带栓菌降解木质纤维素生物质（lignocellulosic biomass）所需水解酶产量的有效策略。