Is it possible to perform bacterial identification and antimicrobial susceptibility testing with a positive blood culture bottle for quick diagnosis of bloodstream infections?

Abstract INTRODUCTION: Bloodstream infections can be fatal, and timely identification of the etiologic agent is important for treatment. METHODOLOGY: An alternative method, consisting of direct identification and susceptibility testing of blood culture bottles using the automated VITEK 2® system, was assessed. RESULTS: All 37 of the Gram-negative bacilli (GNB) identifications and 57.1% of the 28 Gram-positive cocci (GPC) identifications matched those obtained with standard methods. In susceptibility testing, the agreement was greater than 90%. CONCLUSIONS: This alternative methodology may assist in the early identification and susceptibility testing of GNB. Further research is necessary to develop appropriate methods for GPC.

摘要 引言：血流感染可危及生命，及时明确其致病病原体对临床治疗至关重要。
研究方法：本研究评估了一种替代检测方案，即采用自动化VITEK 2®系统对血培养瓶开展直接病原鉴定与药敏试验。
研究结果：37株革兰阴性杆菌（GNB，Gram-negative bacilli）的鉴定结果均与标准方法所得结果一致；28株革兰阳性球菌（GPC，Gram-positive cocci）的鉴定结果中，57.1%与标准方法相符。药敏试验的结果一致性高于90%。
研究结论：该替代检测方法可辅助实现革兰阴性杆菌的早期鉴定与药敏试验。未来仍需开展进一步研究，以开发适用于革兰阳性球菌的标准化检测方法。