Hawaii Island Environmental Sampler Comparison 2016-2018

These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid Ohi'a Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorodi Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 micrometer in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible for Rapid Ohi'a Death (Ceratocystis lukuohia and C. huliohia). Samplers were also tested at a field site with confirmed ROD mortality to demonstrate their ability to detect Ceratocystis DNA in beetle frass from infected trees and wood chips associated with tree felling under a range of environmental conditions. Data files consists of particle counts, particle measurements, and results of qPCR (quantitative polymerase chain reaction) tests to determine presence or absence of Ceratocystis DNA on tape strips that were exposed in samplers and tape strips that were spiked with known numbers of Ceratocystis spores to determine sensitivity of the methodology.

本数据集包含与论文《用于检测引发快速奥希亚死亡的真菌空气传播的经济型环境采样器设计》相关的元数据与关联数据文件。
本数据集共包含8个子数据集，用于2016至2018年间在夏威夷岛开展的受控实验与实地研究，以评估主动式（配备电池驱动风扇）与被动式（依赖风力）美国地质调查局（USGS）环境采样器的应用效果。
实验中同步使用商用采样器Rotorodi Model 20，在受控实验室与实地环境下对上述采样器进行测试，以对比其捕获合成聚乙烯微球（直径12至160微米）与小蠹属（Xyleborus spp.）蛀屑的效能——已知该蛀屑携带引发快速奥希亚死亡的真菌（Ceratocystis lukuohia与C. huliohia）。
此外，研究团队在已确认存在快速奥希亚死亡（ROD）病害致死林木的实地站点开展测试，以验证采样器在多种环境条件下，能否从感病林木的小蠹蛀屑以及伐木相关木片中检测到Ceratocystis属真菌的DNA。
数据文件包含颗粒计数、颗粒粒径测量结果，以及定量聚合酶链式反应（quantitative polymerase chain reaction, qPCR）检测结果，用于判定两类胶带条上是否存在Ceratocystis属真菌的DNA：一类是暴露于采样器中的胶带条，另一类是掺入已知数量该属真菌孢子以验证方法灵敏度的胶带条。