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ORG.one project-Aquilaria crassna -Long-read sequencing data of Aquilaria crassna inferred from ONT platform (MinION). ORG.one project-Aquilaria crassna

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NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB71582
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Rapid sequencing of the critically endangerd Aquilaria crassna Pierre ex Lecomte 1915 as a part of the ORG.one project http://org.one/oo. Aquilaria crassna Pierre ex Lecomte 1915 or agarwood is a member of Thymelaeaceae. This species has been listed as "Critically Endangered - CR" according to The IUCN Red List of Threatened Species because of overexploitation. In this study, we employed the ONT platform (MinION Mk1B device) to sequence the nuclear, mitochondrial, and chloroplast genomes of A. crassna. The total genomic DNA of A. crassna was extracted using a modified CTAB method. Then, the DNA sample was purified using Kit and used for preparing the library using Ligation Sequencing Kit V14 (SQK-LSK 114). The raw data were generated using MinION Mk1B device with R10.4.1 flow cells. Then, the raw data were basecalled using Guppy. There were 2.210.063 reads after basecalling step with HAC option. The length of the reads ranged from 18 bp to 204.287 bp. This dataset is useful for exploring the genomic evolution of A. crassna distributed in Vietnam.
创建时间:
2023-12-27
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