Effect of SB100X-mediated integration on the transcriptome of CD34+ cells

We performed RNA-seq on human CD34+ cells that stably expressed mgmt/GFP transgenes after SB100x-mediated integration and O6BG/BCNU selection in vitro Overall design: CD34+ cells were infected with a HDAd5/35++ vector containing a GFP/mgmt cassette under control of the Ef1a promoter alone or in combination with HDAd-SB. Transduced cells were expanded in erythroid differentation medium for 16 days. Two rounds of O6BG/BCNU selection (50 µM O6BG + 35 µM BCNU) were enriched for GFP-positive cells with integrated transposons. Total RNA were extracted from both samples and subjected to RNA-seq.