Determination of ATP and its metabolites in dietary energy supplements by capillary electrophoresis

Abstract This study aimed to develop a simple and fast capillary electrophoresis (CE) method for the simultaneous determination of adenosine triphosphate (ATP) and its metabolites in dietary energy supplements. Reverse polarity separation mode for faster separation of the three strong negatively charged analytes and capillaries with a 25 µm inner diameter was employed. At -433 V/cm field strength at background electrolyte (BGE) consist with 0.1 M tris-HCl, 0.5 mM tetradecyltrimethylammonium chloride (TTAC) as positively charged surfactant and 0.3 mg/mL hydroxypropylmethylcellulose (HPMC) to reduce the electroosmotic flow (EOF), a complete separation of the three species were achieved in less than 15 minutes. The data acquisition was conducted at a wavelength of 254 nm. Three different commercialised dietary energy supplements were analysed.

摘要 本研究旨在开发一种简便快速的毛细管电泳（CE）方法，用于同时测定膳食能量补充剂中的三磷酸腺苷（ATP）及其代谢物。研究采用反极性分离模式以实现三种强带负电分析物的快速分离，并使用内径为25 µm的毛细管。在场强为-433 V/cm、背景电解质（BGE）由0.1 M三羟甲基氨基甲烷盐酸盐（tris-HCl）、0.5 mM十四烷基三甲基氯化铵（TTAC，带正电的表面活性剂）以及0.3 mg/mL羟丙基甲基纤维素（HPMC，用于降低电渗流（EOF））组成的条件下，仅用不到15分钟即实现了三种分析物的完全分离。数据采集于254 nm波长处完成。本研究对三款商业化膳食能量补充剂进行了分析。