Genome-wide maps at the base-pair level, of chromatin regions enriched for H3 me3 marks in mouse glioma neurospheres (NS)

We generated data from over four billion bases of sequence from chromatin immunoprecipitated using H3K4me3 or H3K27me3 specific antibodies. We generated genome-wide maps of chromatin regions enriched for the two H3 marks described above. Chromatin source were neurospheres harboring mutant or wild-type IDH1. Our data demonstrated that lysine 4 and lysine 27 trimethylation effectively discriminates between genes that are either expressed or repressed. Therefore, reflecting the impact of mIDH1 on the NS transcriptome. Overall design: Examination of 2 different histone modifications in 2 neurospheres types (wt-IDH1 and mIDH1).