Gene expression signature of cerebellum hypoplasia in a mouse model of Down syndrome (Part II)

We designed a large scale gene expression study in cerebellar external granular layer in Ts1Cje mice at P0 in order to measure the effects of trisomy 21 on in a enriched cell population (dissected layer) that is affected in Down syndrome in order to correlate gene expression changes to the phenotype observed. Keywords: Down syndrome, Ts1Cje, EGL, hypoplasia We analyzed gene expression in the EGL of Ts1Cje and euploid mice at P0 using pangenomic Illumina mouse-6 v1.1 expression beadchips containing 46 632 probes representing approximately 19 000 mouse genes. 18 samples from individual cerebellar EGL were hybridized on 18 microarrays (6 by slide). On each slide, we hybridized 6 samples from the same litter.本研究针对出生后0天（Postnatal day 0, P0）的Ts1Cje小鼠小脑外颗粒层（cerebellar external granular layer, EGL）开展大规模基因表达研究，旨在探究21三体（trisomy 21）对唐氏综合征（Down syndrome）受累的富集细胞群体（即经解剖分离的脑组织层）的影响，从而将基因表达变化与观测到的表型建立关联。 关键词：唐氏综合征（Down syndrome）、Ts1Cje、EGL、发育不全（hypoplasia） 我们使用搭载46632个探针、对应约19000个小鼠基因的全基因组Illumina Mouse-6 v1.1版小鼠表达微珠芯片，对P0期Ts1Cje小鼠与整倍体小鼠的EGL组织进行基因表达分析。 本研究共采集18份来自单只小鼠小脑EGL的样本，将其分别点样至18张微阵列芯片（每张芯片点样6份样本）。 每张芯片上的6份样本均来自同窝幼崽。