HER2 aptamer-conjugated iron oxide nanoparticles with PDMAEMA-b-PMPC coating for breast cancer cell identification - supplementary data

<b>Aim: </b>To synthesize HER2 aptamer-conjugated iron oxide nanoparticles with a coating of poly(2- (dimethylamino) ethyl methacrylate)–poly(2-methacryloyloxyethylphosphorylcholine) block copolymer (IONPPPs). <b>Methods: </b>Characterization covered molecular structure, chemical composition, thermal stability, magnetic characteristics, aptamer interaction, crystalline nature and microscopic features. Subsequent investigations focused on IONPPPs for <i>in vitro </i>cancer cell identification. <b>Results: </b>Results demonstrated high biocompatibility of the diblock copolymer with no significant toxicity up to 150 μg<b>/</b>ml. The facile coating process yielded the IONPP complex, featuring a 13.27 nm metal core and a 3.10 nm polymer coating. Functionalized with a HER2-targeting DNA aptamer, IONPPP enhanced recognition in HER2-amplified SKBR3 cells viamagnetization separation. <b>Conclusion: </b>These findings underscore IONPPP’s potential in cancer research and clinical applications, showcasing diagnostic efficacy and HER2 protein targeting in a proof-of-concept approach.

**研究目的：** 旨在合成携聚(2-(二甲氨基)乙基甲基丙烯酸酯)-聚(2-甲基丙烯酰氧乙基磷酰胆碱)嵌段共聚物涂层的HER2适配体（aptamer）偶联氧化铁纳米颗粒（IONPPPs）。**研究方法：** 表征工作涵盖分子结构、化学组成、热稳定性、磁学特性、适配体相互作用、结晶性质及微观形貌特征。后续实验聚焦于IONPPPs的体外（in vitro）癌细胞识别应用。**研究结果：** 结果表明，该二嵌段共聚物具备优异的生物相容性，在浓度高达150 μg/ml时未表现出显著细胞毒性。通过简便的涂层工艺成功制备得到IONPP复合物，其金属核尺寸为13.27 nm，聚合物涂层厚度为3.10 nm。经HER2靶向DNA适配体功能化修饰后，IONPPP可通过磁分离技术增强对HER2过表达SKBR3细胞的识别能力。**研究结论：** 本研究结果证实了IONPPP在癌症研究与临床应用中的潜力，通过概念验证实验展现了其诊断效能与HER2蛋白靶向能力。