Hematological profile of Astyanax bimaculatus under different offer of Lactobacillus sp

ABSTRACT The objective of this study was to evaluate the hematological and immunological parameters of yellowtail lambaris (Astyanax bimaculatus), fed with different frequencies of the probiotic (Lactobacillus spp.). Fishes were distributed into 20 experimental units and divided in five treatments: control (0%), 25%, 50%, 75% and 100% of probiotic supply. A higher presence of total leukocytes (47.70 103cell µl-1), lymphocytes (36.11 103cell µl-1) and monocytes (11.58 103cell µl-1) was verified in fish fed 100% of probiotic, showing a directly proportional ratio among the frequencies of the probiotic supply on the availability of circulating cells in the circulatory system (R² 094-0.97). Hematocrit (27.30-34.63%), hemoglobin (7.00-10.90g dl-1), mean corpuscular volume (4.21-5.45 10-5.pg), mean corpuscular hemoglobin (3.45-5.40 10-6.pg), mean corpuscular hemoglobin concentration, (2.99-4.35g dl-1), total protein (44.32-50.26mg ml-1) and total plasma immunoglobulin (27.96-34.08mg ml-1) did not diverge among treatments. The frequency of the probiotic supply interferes with the hematological profile, although lactic acid bacteria were present in the same concentrations in the intestinal tract, regardless of the probiotic supply, there was an increase in circulating leukocytes, especially lymphocytes and monocytes, in lambari fed probiotic with more frequency.

摘要 本研究旨在评估以不同频率饲喂益生菌（probiotic，乳杆菌属Lactobacillus spp.）的黄尾双斑丽脂鲤（Astyanax bimaculatus）的血液学与免疫学参数。实验鱼被分配至20个实验单元，分为5个处理组：对照组（0%）、25%、50%、75%及100%益生菌投喂组。结果显示，投喂100%益生菌组的鱼体总白细胞（47.70×10³个/微升）、淋巴细胞（36.11×10³个/微升）及单核细胞（11.58×10³个/微升）数量显著更高，且益生菌投喂频率与循环系统中循环血细胞的可用性呈正相关（决定系数R²=0.94~0.97）。各组间的红细胞比容（27.30%~34.63%）、血红蛋白含量（7.00~10.90克/分升）、平均红细胞体积（4.21~5.45×10⁻⁵皮克）、平均红细胞血红蛋白量（3.45~5.40×10⁻⁶皮克）、平均红细胞血红蛋白浓度（2.99~4.35克/分升）、总蛋白含量（44.32~50.26毫克/毫升）及血浆总免疫球蛋白含量（27.96~34.08毫克/毫升）均无显著差异。尽管无论益生菌投喂频率如何，肠道内的乳酸菌浓度均无差异，但本研究表明，益生菌投喂频率会影响血液学指标谱；且随着益生菌投喂频率升高，双斑丽脂鲤的循环白细胞（尤其是淋巴细胞与单核细胞）数量显著升高。