Genomic characterization of Canine circovirus detected in a dog with intermittent hemorrhagic gastroenteritis in Brazil

ABSTRACT: Because Canine circovirus (CanineCV) is a new species of the genus Circovirus, several issues related to its epidemiology, pathogenesis and clinical disease remain unknown. Thus, this study aimed to perform the characterization of the first complete genome sequence of CanineCV detected in a dog with diarrhea in Brazil. A stool sample was collected of a ten-month-old female German Shepherd dog which had signs of intermittent hemorrhagic gastroenteritis, vomiting, and a history of eating raw pork. The complete CanineCV genome was sequenced by Next-Generation Sequencing. The sequence had 2,063 nucleotides, showed a typical genomic organization for circovirus, and was grouped with strain 214 described in the United States by phylogenetic analysis. One amino acid change was found in the replicase protein, and because of that it was considered unique to CanineCV. Therefore, the characterization of the complete genome of Brazilian CanineCV can be used in future studies of molecular epidemiology, pathogenesis and development of diagnostic tools for the prevention and control of this disease.

摘要：犬圆环病毒（Canine circovirus, CanineCV）是圆环病毒属（Circovirus）的新物种，其流行病学、致病机制及临床病症相关的诸多问题尚未明确。为此，本研究旨在解析在巴西腹泻病犬体内首次检出的犬圆环病毒完整基因组序列。研究采集了1只10月龄雌性德国牧羊犬的粪便样本，该犬表现出间歇性出血性胃肠炎、呕吐症状，且有生食猪肉的病史。采用下一代测序（Next-Generation Sequencing）技术完成了该病毒的全基因组测序，其全长为2063个核苷酸，具备圆环病毒典型的基因组结构特征；经系统发育分析（phylogenetic analysis）显示，其与美国报道的214株毒株聚类为同一分支。该病毒的复制酶蛋白（replicase protein）存在1处氨基酸突变，因此被认定为犬圆环病毒的独特毒株。综上，巴西犬圆环病毒全基因组的解析结果，可为后续该病毒的分子流行病学、致病机制研究以及该病防控相关诊断工具的开发提供重要支撑。