Additional file 1 of Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes

Additional file 1. Table S1: Clinical parameters. Table S2: Primers for generating amplicons for targeted bisulfite-sequencing. Table S3: MEST, H19, XIST and DDX4 methylation values obtained by DBS for the testicular sperm samples. Table S4: WGBS statistics. Quality control parameters were based on QualiMap and MethylDackel reports. Table S5: WGBS methylation values for the 50 imprinting controls regions. Table S6: High-confidence TGC unmethylated regions. Table S7: High-confidence TGC low-methylated regions. Table S8: WGBS methylation values for the 1329 DMRs. Table S9: Annotations for the 271 CTR-CZ DMRs. Table S10: DMR-associated genes. Table S11: Differentially expressed DMR-associated genes using tradeSeq. Table S12: Differentially expressed DMR-associated genes using MAST.

附加文件1。表S1：临床参数。表S2：用于制备靶向亚硫酸氢盐测序（targeted bisulfite-sequencing）扩增子的引物。表S3：通过DBS（Direct Bisulfite Sequencing）获取的睾丸精子样本中MEST、H19、XIST及DDX4的甲基化水平数值。表S4：全基因组亚硫酸氢盐测序（WGBS）统计数据。质控参数基于QualiMap与MethylDackel的分析报告。表S5：50个印记调控区域的WGBS甲基化水平数值。表S6：高置信度TGC非甲基化区域。表S7：高置信度TGC低甲基化区域。表S8：1329个差异甲基化区域（DMRs，Differentially Methylated Regions）的WGBS甲基化水平数值。表S9：271个CTR-CZ DMRs的注释信息。表S10：与DMR相关的基因。表S11：采用tradeSeq分析得到的差异表达DMR相关基因。表S12：采用MAST分析得到的差异表达DMR相关基因。