Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain – Del/+-Del/+ - 5224_5173

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.

本研究评估了成对陌生个体间的社会互动与交流行为。针对此类社会行为与超声通讯的录制工作，由于当动物近距离互动时当前无法识别超声发声信号（USVs）的发出者，因此本研究将观察对象限定为成对个体。为此，本研究对来自不同饲养笼的同周龄（14至20周龄）、同基因型的两只陌生个体之间的无干扰成对互动进行了为期47小时（两昼夜）的录制。为实现上述目标，本研究将活体小鼠追踪器（Live Mouse Tracker）系统（插件931）与一台CM16/CMPA麦克风（Avisoft Bioacoustics，德国格利尼克）相连，该麦克风接入Avisoft Ultrasound Gate 416设备（采样率300 kHz，16位格式；触发条件：该通道电平；预触发时长1秒；保持时长1秒；有效信号时长>0.005秒；触发判定规则：25-125 kHz频段内能量占比2%且熵值<50%；Avisoft Bioacoustics，德国格利尼克）。活体小鼠追踪器（LMT）与Avisoft系统依据de Chaumont等人2021年发表的实验方案完成了同步校准。每份上传文件均包含来自LMT的SQLite数据库（已完成处理并完成USVs同步），以及一个包含Avisoft设备自动录制的全部USVs序列的压缩包。

搜集汇总

背景与挑战

背景概述

该数据集记录了16p11.2缺失小鼠品系（Del/+-Del/+基因型）中陌生雌性小鼠对在两天两夜（47小时）内的社交互动和超声通信数据。实验使用Live Mouse Tracker系统和Avisoft超声设备同步记录行为数据，旨在评估个体间的社交行为和通信模式，数据包括处理后的LMT数据库和自动记录的超声发声序列文件。