METHODS AND COMPOSITIONS FOR SHORT STATURE PLANTS THROUGH MANIPULATION OF GIBBERELLIN METABOLISM TO INCREASE HARVESTABLE YIELD

The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression， mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics， such as reduced plant height and increased lodging resistance， but without off-types.

本公开提供了用于调控玉米或其他谷类植物体内赤霉素（gibberellin，GA）含量的组合物与方法。本公开还提供了通过抑制、诱变和/或编辑GA20氧化酶（GA20 oxidase）或GA3氧化酶（GA3 oxidase）基因的特定亚型，以调控赤霉素生物合成相关基因表达的组合物与方法。本公开进一步提供了经修饰的植物细胞与植物：其携带可降低GA氧化酶基因表达或活性的抑制元件或突变，能够实现赤霉素水平降低且性状得到改良——例如株高降低、抗倒伏性提升，且不会产生非预期异型株（off-types）。