Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR

Abstract INTRODUCTION Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. METHODS This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. RESULTS: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. CONCLUSIONS: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols.

摘要 引言：病原体检测分子技术已被证实为兼具高灵敏度与短检测周期的高效诊断工具。方法：本研究对比了五种用于聚合酶链式反应（PCR）检测的金黄色葡萄球菌（Staphylococcus aureus）DNA提取方法。结果：各方法提取所得DNA的浓度与纯度均未达到优异质量水平，但多数实验方案即便在DNA浓度较低的情况下，仍可实现100%的阳性检出率。结论：尽管其中一种实验方案较其余方案更为高效，但所有实验方案均可依托PCR的高灵敏度完成金黄色葡萄球菌的检测。