Expression profile of RCC4 cells with TAZ (WWTR1) silencing

TAZ, also known as WWTR1, is the one of the effectors of Hippo pathway. With its paralog, YAP, TAZ promotes organ size growth as well as tumor metastasis. In human renal carcinoma cells, we found that TAZ-silencing induces resisntance toward erastin-induced ferroptosis. In this study, TAZ is silencing in clear cell carcinoma cell line RCC4 to elucidate the downstream targets that promotes the resistance toward erastin-induced ferroptosis. Human clear cell renal carcinoma cell line RCC4 were transfected with siRNA targeting TAZ (siTAZ) and compared with non-targeting siRNA control (siNT). All samples were prepared as biological triplicates. Total RNA was collected after 2 days of reverse transfection for cDNA synthesis. The influence of silencing TAZ on gene expression was derived by zero transformation process, in which we compared the gene expression level with siTAZ to the average of gene expression level in control (siNT) samples.