MCR LTER: Coral Reef: Conspecific aggregation mitigation of OA on calcification of the coral Pocillopora verrucosa, JEXBIO 2017

The study was conducted in April 2015 in Moorea, French Polynesia, using colonies of Pocillopora verrucosa (~ 4 cm in planar diameter) collected from the outer reef of the north shore at 10–12 m depth.
Corals were collected from multiple sites separated by 100-200 m on the outer reef to maximize the likelihood that the selected coral colonies were genetically unique, and transferred directly to an acclimation tank.
The experiment used a sequential design, in which corals first were incubated under 130 ambient or elevated pCO 2 in flow-through tanks, and then were incubated in a recirculating flume under the same pCO 2 crossed with a contrast of two colony densities (Fig. S1). Two
response variables were measured in the light (calcification and net photosynthesis at a single irradiance), two response variables were measured in the dark (aerobic respiration and calcification), and two response variables were calculated from these values (gross
135 photosynthesis, and calcification integrated over 24 h). Aerobic respiration was measured as oxygen uptake, and net photosynthesis was measured as the flux of oxygen at a constant irradiance, and in both cases, oxygen uptake was given a negative notation and oxygen evolution a positive notation; gross photosynthesis was obtained by subtracting respiration from net photosynthesis. Daily calcification was calculated by integrating calcification in the
140 light over 12 h, calcification in the dark over 12 h, and summing the two values assuming each day consisted of 12 h of light at a constant intensity. The six response variables were measured for aggregates of a fixed number (n = 12) of similar-sized colonies placed in the flume in either high or low density arrays. With this design, it was not possible to measure the physiology of individual colonies in each aggregate, and therefore our results describe the
145 performance corals averaged across each aggregate.
These data support the publication Evensen & Edmunds,
'Conspecific aggregations mitigte the effects of ocean acidification on calcification of the coral Pocillopora verrucosa ', JEXBIO 2017, doi:10.1242/jeb.152488. This material is based upon work supported by the U.S. National Science Foundation under
Grant No. OCE 16-37396 (and earlier awards) as well as a generous gift from the Gordon and
Betty Moore Foundation. Research was completed under permits issued by the French Polynesian
Government (Délégation à la Recherche) and the Haut-commissariat de la République en
Polynésie Francaise (DTRT) (Protocole d'Accueil 2005-2018). This work represents a
contribution of the Moorea Coral Reef (MCR) LTER Site.

本研究于2015年4月在法属波利尼西亚的莫雷阿岛（Moorea）开展，实验所用的疣杯孔珊瑚（Pocillopora verrucosa）菌落（平面直径约4 cm）采自北外礁10–12 m水深区域。珊瑚采自外礁上间距100-200 m的多个采样点，以最大化所选珊瑚菌落的遗传唯一性，随后直接转移至驯化水箱。本实验采用序贯设计：珊瑚首先在流通式水箱中于环境水平或升高的pCO₂（二氧化碳分压）条件下培养，随后在循环水槽中于相同pCO₂条件下结合两种菌落密度梯度进行培养（图S1）。本研究共测定六项响应变量：光照条件下测定钙化速率与单次辐照度下的净光合速率；黑暗条件下测定有氧呼吸速率与钙化速率；另外两项响应变量由上述测定值计算得到：总光合速率，以及24小时积分钙化速率。有氧呼吸以氧气摄取量表征，净光合速率以恒定辐照度下的氧气通量表征，两类指标均以氧气摄取记为负值、氧气释放记为正值；总光合速率通过净光合速率减去呼吸速率计算得到。每日钙化速率通过将12小时光照时段的钙化速率、12小时黑暗时段的钙化速率分别积分后求和得到，假设每日为恒定辐照度的12小时光照与12小时黑暗交替。六项响应变量针对固定数量（n=12）的同规格菌落聚集体测定，这些聚集体以高密度或低密度阵列放置于水槽中。由于该实验设计无法测定每个聚集体中单株珊瑚的生理状态，因此本研究结果为各聚集体的珊瑚平均表现。本数据集支撑Evensen与Edmunds于2017年发表于JEXBIO的论文《同种聚集体缓解海洋酸化对疣杯孔珊瑚钙化作用的影响》，DOI:10.1242/jeb.152488。本研究工作得到美国国家科学基金会（U.S. National Science Foundation）编号OCE 16-37396的项目资助（含前期资助项目），以及戈登与贝蒂·摩尔基金会（Gordon and Betty Moore Foundation）的慷慨捐赠。研究开展获得法属波利尼西亚政府（Délégation à la Recherche）及法国驻法属波利尼西亚高级专员公署（Haut-commissariat de la République en Polynésie Francaise，缩写DTRT）颁发的许可（Protocole d'Accueil 2005-2018）。本研究为莫雷阿岛珊瑚礁（MCR）长期生态研究（LTER）站点的成果贡献。