Figure 3C

<b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.

<b>A. </b>转染空载体或UL88的MRC-5细胞经二甲基亚砜（DMSO）、MG132（10 mM）或氯喹（Chloroquine，50 mM）处理。收集细胞裂解液进行免疫印迹（western blotting）分析，以UL88、髓系分化因子88（MyD88）及p115（加载对照）为探针进行抗体杂交，展示代表性免疫印迹结果。<b>B. </b>本图为三次独立重复实验的定量统计结果，实验方法如<b>A</b>所述。<b>C. </b>293细胞共转染HA标记的MyD88与空GFP载体、全长GFP-UL88、GFP-UL88 C端片段或GFP-UL88 N端片段。转染48小时后收集细胞裂解液，经免疫印迹分析，以HA、GFP及p115（加载对照）为探针进行抗体杂交。<b>D.</b> 293细胞共转染Flag标记的MyD88与全长GFP-UL88、GFP-UL88 C端片段或GFP-UL88 N端片段。转染48小时后收集细胞裂解液，使用磁珠偶联的抗Flag抗体进行免疫共沉淀（co-immunoprecipitation）实验。样品经免疫印迹分析，以GFP、Flag及p115（加载对照）为探针进行抗体杂交。免疫沉淀印迹中的箭头指向GFP-UL88、GFP-UL88 N端片段及Flag-MyD88条带。