Expression data from Shp2f/f and SCSKO mice testis

The ability of male reproduction is seriously dependent on Sertoli cells. However, the mechanisms governing the functional integrity of Sertoli cells remained largely unexplored. Tyrosine phosphatase protein Shp2 is expressed in germ, Leydig and Sertoli cells of mice testes. But the physiological role of Shp2 in the spermatogenesis was not fully understood. Thus, we conditionally deleted Shp2 gene in Sertoli cells using two transgenic models, and demonstrated that Shp2 deficiency caused infertility, excessive differentiation of SSCs and abnormal BTB in mice. To further discover the underlying mechanism of Shp2 regulation, we collected the mRNA of testes from wild type or knockout mice at 16.5 fetal day, Postnatal 3 days, 1 weeks, 2 weeks, and then screened the gene expression. Testis tissues from Shp2f/f and SCSKO mice were for RNA extraction and hybridization on Affymetrix microarrays.雄性生殖能力高度依赖于支持细胞（Sertoli cells）。然而，维持支持细胞功能完整性的分子机制在很大程度上仍未被阐明。酪氨酸磷酸酶蛋白Shp2（Tyrosine phosphatase protein Shp2）在小鼠睾丸的生殖细胞、莱迪希细胞（Leydig cells）以及支持细胞中均有表达，但Shp2在精子发生过程中的生理功能尚未完全明确。因此，我们通过两种转基因模型在支持细胞中条件性敲除Shp2基因，实验结果表明，Shp2缺失会导致小鼠不育、精原干细胞（SSCs，Spermatogonial Stem Cells）过度分化以及血睾屏障（BTB，Blood-Testis Barrier）异常。为了进一步探究Shp2调控的潜在分子机制，我们采集了胎龄16.5天、出生后3天、1周及2周的野生型与敲除型小鼠的睾丸组织mRNA，随后开展基因表达筛选。我们对Shp2f/f与SCSKO小鼠的睾丸组织进行RNA提取，并在Affymetrix基因芯片上完成杂交实验。