Supporting Information files.rar

Spinal cord injury (SCI) leads to severe vascular disruption, hypoxia, and tissue damage, significantly impairing functional recovery. This study investigates the role of hypoxia-treated in promoting angiogenesis and tissue repair following SCI, focusing on the HIF-1 and PI3K/AKT signaling pathways. Transcriptomic analysis revealed significant activation of angiogenesis-related genes, including Vegfa, Igf1, and Angpt2, in the hypoxia-treated group. Functional experiments demonstrated that hypoxia-treated enhanced vascular regeneration, increased endothelial cell density, and improved tissue repair, mediated through the activation of PI3K/AKT and HIF-1 pathways. Inhibition of PI3K/AKT signaling significantly attenuated these effects, confirming its critical role in hypoxia-induced angiogenesis. These findings provide insights into the molecular mechanisms of hypoxia-induced repair and support the development of targeted therapies to enhance SCI recovery.