COMPARISON OF EFFICIENCY OF STAINING WITH ZIEHL-NEELSEN MODIFIED AND SAFRANIN MODIFIED IN DETECTION OF Cryptosporidium spp. (EUCOCCIDIORIDA, CRYPTOSPORIDIIDAE) FROM FECAL SAMPLES OF CALVES FROM 0 AND 3 MONTHS

Abstract Bovine cryptosporidiosis is caused by four differents species: Cryptosporidium parvum, Cryptosporidium bovis, Cryptosporidium andersoni and Cryptosporidium ryanae. The species Cryptosporidium parvum (Order: Eucoccidiorida, Family: Cryptosporidiidae) is considered of high zoonotic potential and it can infect humans through the elimination of oocysts by both cattle and by humans. The objective of this research was to detect oocysts of the genus Cryptosporidium spp. in fecal contents of calves (75 males and 77 females). We collected 152 stool samples from animals aged between 0 day and 3 months. The material was subjected to modified Ziehl-Neelsen and modified Safranin techniques, the slides were observed in its entire length by optical microscopy to verify the presence of oocysts of this parasitic infections. The results showed 17.1% (26/152) positivity in the samples examined, and the statistical analysis showed no difference between sex and the staining techniques used in this study. We concluded the infection by Cryptosporidium spp. is present in the evaluated properties, but more studies are needed, so that the risk of infection is measured properly and prophylactic measures are implemented.

摘要 牛隐孢子虫病由四种不同隐孢子虫属物种引发：小隐孢子虫（Cryptosporidium parvum）、牛隐孢子虫（Cryptosporidium bovis）、安氏隐孢子虫（Cryptosporidium andersoni）以及瑞安隐孢子虫（Cryptosporidium ryanae）。其中小隐孢子虫（目：真球虫目，科：隐孢子虫科）被认为具备高人畜共患潜能，可通过牛与人类排出的卵囊感染人类。本研究旨在对犊牛（75头雄性、77头雌性）的粪便内容物中的隐孢子虫属（Cryptosporidium spp.）卵囊进行检测。我们从0日龄至3月龄的动物中采集了152份粪便样本，对样本分别采用改良齐尔-尼尔森染色法（modified Ziehl-Neelsen）与改良番红染色法（modified Safranin）进行处理，随后通过光学显微镜对玻片全区域进行观察，以确认该寄生虫感染卵囊的存在情况。结果显示，受检样本的阳性率为17.1%（26/152）；统计学分析表明，性别与本研究使用的染色技术之间均无显著差异。本研究得出结论：隐孢子虫属感染在被评估的养殖场地中普遍存在，但仍需开展更多研究，以准确评估感染风险并落实预防措施。