Effect of using inoculant on elephant grass silage with additives

ABSTRACT. This study aimed to evaluate the use of inoculant on silage of elephant grass, wet brewery waste and buriti meal at different silo opening times. The experimental silage tested was composed of 50% elephant grass, 30% wet brewery waste, and 20% buriti meal. The experimental design was randomized block in a factorial arrangement (2 x 4), where the treatments consisted of the use or not of inoculant during silage processing, and four silo opening times (7, 14, 21, and 28 days). Data collected were firstly subjected to ANOVA and subsequent Tukey’s test. Results were considered significant at p ≤ 0.05. The use of inoculant in silage production provided more acidic pH (p < 0.05) and higher (p < 0.05) effluent losses. Longer silo opening times resulted in more alkaline pH (p < 0.05) and higher (p < 0.05) effluent losses. The use of inoculant in silage production and longer silo opening times provided higher (p < 0.05) content of ash, crude protein, and fats, and lower (p < 0.05) content of dry matter and fiber (neutral and acid detergent). However, there was no interaction between the factors evaluated in this study. It was concluded that the inoculant may be used in the production of elephant grass silages, wet brewery waste, and buriti meal, providing a more acidic pH, higher effluent losses, lower contents of dry matter and fiber, and higher nutritional content. As the opening time was extended up to 28 days, there was more alkaline pH, higher effluent losses, lower dry matter and fiber content, and higher nutritional content.

摘要。本研究旨在评估青贮接种剂（inoculant）在不同开封时长下，应用于象草（elephant grass）、啤酒湿糟（wet brewery waste）及布里蒂粕（buriti meal）混合青贮的效果。试验青贮的原料配比为50%象草、30%啤酒湿糟与20%布里蒂粕。本试验采用2×4析因设计的随机区组试验，处理组包含两类：一是青贮制作过程中是否添加青贮接种剂，二是4个青贮窖开封时间（7、14、21及28天）。试验采集的数据首先进行方差分析（ANOVA），随后采用Tukey法进行多重比较，以p≤0.05作为差异显著性判定标准。

青贮接种剂的使用可使青贮pH值更趋酸性（p<0.05），同时提升渗出液损失量（p<0.05）；延长青贮窖开封时间则会使青贮pH值更趋碱性（p<0.05），并提高渗出液损失量（p<0.05）。此外，青贮接种剂的使用与更长的开封时间，均可显著提高青贮灰分、粗蛋白及粗脂肪含量（p<0.05），同时降低干物质与中性、酸性洗涤纤维含量（p<0.05）。但本研究涉及的两个试验因素未产生显著交互作用。

综上，青贮接种剂可应用于象草、啤酒湿糟及布里蒂粕的混合青贮生产，能够使青贮pH值更趋酸性、提升渗出液损失量，降低干物质与纤维含量，同时提高营养物质含量。随着青贮窖开封时间延长至28天，青贮pH值逐渐偏碱性、渗出液损失量升高，干物质与纤维含量降低，营养物质含量亦随之提升。