2017-2020年吉林长春大熊猫源细小病毒病毒样颗粒构建数据集
收藏国家农业科学数据中心2021-10-15 更新2024-03-07 收录
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将大熊猫源细小病毒VP2基因优化后克隆至pFastBac Dual载体，同源重组获得穿梭质粒rBacmid-Panda-dVP2，转染Sf9细胞拯救获得表达大熊猫源细小病毒VP2蛋白的重组杆状病毒rpFBD-Panda-dVP2，重组病毒感染昆虫细胞后可成功组装成病毒样颗粒；大熊猫源细小病毒VLPs免疫幼猫后可诱导机体产生高效价血凝抑制抗体，且保护性抗体水平可持续至少12个月。大熊猫源细小病毒VLPs的成功构建,为大熊猫细小病毒病新型基因工程疫苗的研制奠定了基础，对防控大熊猫细小病毒病具有重要意义。
The optimized VP2 gene of giant panda-derived parvovirus was cloned into the pFastBac Dual vector, and the shuttle plasmid rBacmid-Panda-dVP2 was obtained through homologous recombination. Sf9 cells were transfected with this shuttle plasmid to rescue the recombinant baculovirus rpFBD-Panda-dVP2, which expresses the VP2 protein of giant panda-derived parvovirus. When the recombinant baculovirus infects insect cells, virus-like particles (VLPs) can be successfully assembled. Immunization of kittens with these giant panda-derived parvovirus VLPs induced the production of high-titer hemagglutination inhibition (HI) antibodies, and the levels of protective antibodies persisted for at least 12 months. The successful construction of the giant panda-derived parvovirus VLPs laid a solid foundation for the development of novel genetic engineering vaccines against giant panda parvovirus disease, and is of great significance for the prevention and control of this disease.
提供机构：
长春西诺生物科技有限公司
创建时间：
2021-10-15
