circRNA and mRNA transcriptome sequencing data from OVA-induced mouse lung tissue

<b>Chronic Allergic Pulmonary Inflammation Model</b>The male C57BL/6J mice utilized in this study were six weeks old, weighed 22±2 grams. The mice were allocated into four distinct groups, each consisting of six animals: the control group, the asthma group, the exercise group, and the asthma + exercise group. The asthma group and asthma+ exercise group were administered aluminum hydroxide-diluted OVA at a dose of 10 μg per mouse via intraperitoneal injection on days 0, 14, 28, and 42, following a previously established protocol. From day 21 to day 54, the mice in these groups were exposed to a 1% OVA aerosol for 30 minutes, three times per week. In contrast, the mice in the control group and the exercise group were exposed to saline under the same conditions. Mice are anesthetized with 1% pentobarbital sodium via intraperitoneal injection. Euthanasia is performed by tail vein injection of a 3% pentobarbital sodium solution.<b>RNA Sequencing</b>Shanghai Biotechnology Corporation conducted the RNA microarray analysis. Total RNA was extracted from the lung tissues of three OVA-induced mice and three healthy control mice via the RNeasy Mini Kit (Qiagen). For RNA-seq, strand-specific libraries were prepared via the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina). the samples were quantified via a Qubit 2.0 fluorometer (Life Technologies), and the insert size was verified via a 2100 Bioanalyzer (Agilent). Following the dilution of the library to 10 pM, clustering was achieved via cBot, and sequencing was conducted on the Illumina NovaSeq 6000 platform. Shanghai Biotechnology Corporation was responsible for both library construction and sequencing.

**慢性变应性肺部炎症模型**
本研究使用的雄性C57BL/6J小鼠为6周龄，体重为22±2克。将小鼠分为4个独立组别，每组各6只：对照组、哮喘模型组、运动干预组以及哮喘+运动干预组。参照已发表的实验方案，哮喘模型组与哮喘+运动干预组于第0、14、28、42天，以每只小鼠10μg的剂量腹腔注射氢氧化铝佐剂稀释的卵清蛋白（OVA）。自第21天至第54天，上述两组小鼠每周三次暴露于1%OVA气溶胶中，每次暴露时长30分钟。与之相对，对照组与运动干预组小鼠在相同条件下仅暴露于生理盐水。实验中以1%戊巴比妥钠腹腔注射对小鼠进行麻醉，通过尾静脉注射3%戊巴比妥钠溶液实施安乐死。

**RNA测序**
上海生物科技有限公司负责本次RNA芯片分析。采用RNeasy Mini试剂盒（Qiagen）从3只OVA诱导模型小鼠与3只健康对照小鼠的肺组织中提取总RNA。对于RNA测序（RNA-seq），采用TruSeq Stranded Total RNA样本制备试剂盒（Illumina）构建链特异性文库。通过Qubit 2.0荧光计（Life Technologies）对样本进行定量，并使用2100生物分析仪（Agilent）验证插入片段大小。将文库稀释至10pM后，通过cBot完成簇生成，随后在Illumina NovaSeq 6000测序平台上进行测序。文库构建与测序工作均由上海生物科技有限公司完成。