Metadata Entered in Submitting Proteomic Data to PRIDE

For the identification of potential pathways and their interactions that underlie a complex change in cellular state, such as neurite outgrowth, we stimulated the neuroblastoma cell line N2A with HU210, a potent activator of the cannabinoid receptor type I, followed by transcriptomic and proteomic analysis. Differentially expressed genes and proteins obtained at multiple timepoints were subjected to standard and dynamic enrichment analysis using the Molecular Biology of the Cell Ontology. Many of the identified pathways described constitutive cellular functions. Involvement of predicted pathways in neurite outgrowth was confirmed by siRNA knock-down of pathway representative genes in primary rat cortical neurons. Dynamic modeling of pathway activities suggested that the identified increase in vesicle membrane back-transported from the growth cone to the cell body ensures recycling of components needed by forward moving vesicle.