Additional file 1 of Natrix: a Snakemake-based workflow for processing, clustering, and taxonomically assigning amplicon sequencing reads

Additional file 1. Example configuration file with default values. For the configuration of sub-workflows and applications used by Natrix, a single configuration file is used. Optional parts of the workflow (e.g., the generation of quality reports, clustering of OTUs, or the assignment of taxonomic information) can be disabled. The configuration file is also used to define the protocols used to generate the input data, e.g., whether the data is in single-end or paired-end format and whether a split-sample approach was used during sample preparation. It also contains configuration options to adjust individual parts of the workflow depending on the requirements of the project. An example configuration file with a description and default value for each parameter is shown in the CSV file.

附加文件1：附带默认参数值的示例配置文件。针对Natrix所使用的子工作流与应用程序的配置，仅需单个配置文件即可完成。工作流中的可选模块（例如质量报告生成、操作分类单元（Operational Taxonomic Units, OTUs）聚类、分类学信息注释）均可选择禁用。该配置文件还可用于定义生成输入数据所遵循的实验方案，例如数据采用单端（single-end）还是双端（paired-end）测序格式，以及样本制备阶段是否采用了拆分样本（split-sample）策略。同时，配置文件还包含可根据项目需求调整工作流各模块的参数选项。本CSV文件中附带了一份示例配置文件，其中对每个参数均附有详细说明与默认参数值。