Computational IHC-H&E mapping in mouse models of colitis (H&E WSIs from paired H&E-IHC WSIs)

Inflammatory Bowel Disease (IBD) is characterized by chronic, dysregulated inflammation in the gastrointestinal tract. The heterogeneity of IBD is reflected through two major subtypes, Crohn’s Disease (CD) and Ulcerative Colitis (UC). CD and UC differ across symptomatic presentation, histology, immune responses, and treatment. While colitis mouse models have been influential in deciphering IBD pathogenesis, no single model captures the full heterogeneity of clinical disease. The translational capacity of mouse models may be augmented by shifting to multi-mouse model studies that aggregate analysis across various well-controlled phenotypes. Here, we evaluate the value of histology in multi-mouse model characterizations by building upon a previous pipeline that detects histological disease classes in hematoxylin and eosin (H&E)-stained murine colons. Specifically, we map immune marker positivity across serially-sectioned slides to H&E histological classes across the dextran sodium..., , The slides are multi-resolution and openable utilizing WSI viewing software, such as QuPath. Data can also be accessed using standard Python libraries like Openslide.Â

炎症性肠病（Inflammatory Bowel Disease, IBD）以胃肠道慢性、失调性炎症为典型特征。该疾病的异质性主要体现为两大亚型：克罗恩病（Crohn’s Disease, CD）与溃疡性结肠炎（Ulcerative Colitis, UC）。二者在症状表现、组织学特征、免疫应答及治疗策略上均存在显著差异。尽管小鼠结肠炎模型在解析IBD发病机制方面发挥了重要作用，但尚无单一模型能够涵盖临床疾病的全部异质性。若转向开展整合多种表型可控模型的联合分析研究，可有效提升小鼠模型的转化应用能力。

本研究基于此前用于检测苏木精-伊红（hematoxylin and eosin, H&E）染色小鼠结肠组织学疾病类别的分析流程，评估组织学特征在多小鼠模型表征中的应用价值。具体而言，本研究将连续切片玻片上的免疫标记物阳性表达情况，对应至右旋糖酐硫酸钠（dextran sodium...）诱导的H&E组织学分类中。

该玻片为多分辨率格式，可通过全视野数字切片（Whole Slide Image, WSI）浏览软件（如QuPath）打开。该数据集也可通过Openslide等标准Python库进行读取。