Data from: The ORFIUS complex regulates ORC2 localization at replication origins (Flow cytometry)

In this work, we use bromodeoxyuridine (BrdU)/propidium iodide cell cycle flow cytometry profiling to demonstrate that treatment of ovarian cancer cell lines with gene specific siRNAs, shRNAs, or drugs leads to minimal or no cell cycle arrest which might otherwise influence observed phenotypes. All raw FCS files for flow cytometry data presented in Figures 4E, 6G, S3A, S4C, S5B, S5F, S8C, S8D, S8E, S9C, S10C, and S12B are available here.

本研究采用溴脱氧尿苷（bromodeoxyuridine, BrdU）/碘化丙啶（propidium iodide）进行细胞周期流式细胞术分析，结果证实：使用基因特异性小干扰RNA（small interfering RNA, siRNA）、短发夹RNA（short hairpin RNA, shRNA）或药物处理卵巢癌细胞系时，仅会引发极轻微的细胞周期阻滞，或几乎不会引发此类阻滞——而该类阻滞本可能对所观测到的表型产生影响。本研究中图4E、6G、S3A、S4C、S5B、S5F、S8C、S8D、S8E、S9C、S10C及S12B所展示的流式细胞术数据对应的全部原始FCS文件，均可在此处获取。