Resource competition drives an invasion-replacement event among shrew species on an island

Invasive mammals are responsible for the majority of native species' extinctions on islands. While most of these extinction events will be due to novel interactions between species (e.g. exotic predators and naive prey), it is more unusual to find incidences where a newly invasive species cause the decline/extinction of a native species on an island when they normally coexist elsewhere in their overlapping mainland ranges.
We investigated if resource competition between two insectivorous small mammals was playing a significant role in the rapid replacement of the native pygmy shrew (Sorex minutus) in the presence of the recently invading greater white-toothed shrew (Crocidura russula) on the island of Ireland.
We used DNA metabarcoding of gut contents from >300 individuals of both species to determine each species’ diet and measured the body size (weight and length) during different stages of the invasion in Ireland (before, during, and after the species come into contact with one an..., This is a metabarcoding dataset for Browett, S.S., Synnott, R., O'Meara, D., Antwis, R.E., Browett, S.S., Bown, K., Wangensteen, O., Dawson, D.A., Searle, J.B., Yearsley, J.M. and McDevitt, A., 2022. Resource competition drives an invasion-replacement event among shrew species on an island. bioRxiv. doi: https://doi.org/10.1101/2022.04.27.489660
Briefly, DNA was extracted from the gut contents of >300 shrews (Sorex minutus and Crocidura russula). The COI region was amplified using a primer set referred to as Gillet. There are eight multiplexed fastq files from each of the two sequencing runs. Four forward read files and four reverse read files for each run. Samples were multiplexed using primers with dual-index 8 base pair MID tags on them. The sequences contain the primer region and MID tags. A file with the list of tags and primer sequence is included. Sequencing adapters were ligated using a PCR-free method. The final library with a total of 303 samples, five extraction blanks, an..., The list containing the MID tags and individual sample names for each library are included. The columns are
1: Primer used
2: Sample ID
3: Forward MID tag
4: Reverse MID tag
5: Forward primer sequence
6: Reverse primer sequence
7:9 misc info required for pipeline used for the article.

入侵哺乳动物是导致岛屿本土物种绝灭的主要诱因。此类绝灭事件多源于物种间的新型相互作用（如外来捕食者与缺乏反捕食经验的猎物），但在岛屿系统中，当某一新近入侵物种与本土物种在二者重叠的大陆分布区内原本可稳定共存时，该入侵物种却引发本土物种衰退或绝灭的案例则较为罕见。
本研究针对爱尔兰岛上的两种食虫小型哺乳动物展开探究，旨在明确在新近入侵的大麝鼩（Crocidura russula）存在的背景下，二者间的资源竞争是否在本土小鼩鼱（Sorex minutus）的快速替代过程中发挥了关键作用。
我们对两种物种共300余只个体的肠道内容物开展DNA元条形码（DNA metabarcoding）分析，以确定各自的食性；同时在爱尔兰岛入侵进程的不同阶段（两物种接触前、接触期间及接触后）测量了个体的体型（体重与体长）。本数据集对应论文：Browett, S.S.、Synnott, R.、O'Meara, D.、Antwis, R.E.、Browett, S.S.、Bown, K.、Wangensteen, O.、Dawson, D.A.、Searle, J.B.、Yearsley, J.M. 与 McDevitt, A. 于2022年发表于预印本平台bioRxiv的研究《资源竞争驱动岛屿上鼩鼱物种的入侵替代事件》（原文标题：Resource competition drives an invasion-replacement event among shrew species on an island），DOI：https://doi.org/10.1101/2022.04.27.489660。
简言之，我们从300余只小鼩鼱与大麝鼩的肠道内容物中提取基因组DNA。以命名为Gillet的引物组扩增COI区域。本次测序共设置两轮，每轮均生成8个多重混合的FASTQ文件，其中正向读段文件与反向读段文件各4个。实验采用带有双索引8碱基MID标签（MID tag）的引物对样本进行多重标记，测序序列包含引物区域与MID标签序列，配套提供了包含标签与引物序列列表的文件。测序接头采用无PCR方法进行连接。最终构建的文库共包含303个样本、5个提取空白对照，[原文此处存在内容省略]。本数据集附带各文库的MID标签与单个样本名称列表，其列信息如下：
1. 所用引物
2. 样本ID
3. 正向MID标签
4. 反向MID标签
5. 正向引物序列
6. 反向引物序列
7至9：本文所用生物信息学分析流程所需的其他杂项信息。