Spatial Transcriptomics Reveals Expression Gradients in Developing Wheat Inflorescences at Cellular Resolution

Bulk RNA-sequencing of microdissected wheat spike meristems at five stages of early development. Meristems were dissected into basal and central sections and pooled to produce individual samples. This dataset was used to identify genes that were differentially expressed between the basal and central portions of wheat spike meristems at various stages during early development. This analysis then informed the selection of a 200 gene panel for which we conducted MERFISH (Multiplexed Error Robust Fluorescence In Situ Hybridization) spatial transcriptomics on similar wheat meristem tissues.