Data presented in Brinkmann et al., 2021, Aquat. Toxicol.
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These files include all data presented in: Brinkmann et al., 2021. Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota. Aquatic Toxicology. 16SrRNA_(...) fasta and fastq formatted 16SrRNA sequences (Fig. 3c/ Table S4) with in the description line: * Colony numbers corresponding to the numbers included in Table S4 * The applied sequencing primer (27F) * NP exposure: nTiO2 (5 mg/L) or Control (no NPs) * A code referring to the parental zebrafish family that was crossed to obtain the larvae (A7, A8, or A9) 2photon_(...) * Two-photon scans of Fig. 2 * ROI sets of Fig. 2b/e (zipped) * Wavelength and focus information (.dat files) CLSM_(...) * CLSM scans of Fig. 3b (zipped) * ROI (zipped) * Metadata, where scanID corresponds to scan and ROI numbers of the zipped data CFUcounts_(...) CFU count data (Fig. 4/ Fig. S7): * Compound: Exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or control (no NPs) * LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf) * Date: Date of CFU isolation * DilutionFactor: Counted dilution * CFU_Larvae: CFU count per egg or larva DLS_(...) DLS measurements for nTiO2 (Table 2): * Time: Incubation time (h) * Concentration: Nominal concentration (mg/L) * ZP: Zeta potential (mV) * d_size: Z-averag size (nm) DoseResponses_(...) Mortality data of zebrafish larvae exposed to nTiO2 from 3-5 dpf (Fig. S6): * Concentration: Nominal exposure concentration (mg/L) * Day: Exposure time (days). * Mortality: Percentage of larvae that had died * Date: The date at which the mortality was scored EcoPlate_(...) EcoPlate measurement data (Fig. 5/ S3/ S8/ S9): * Compound: Zebrafish egg exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or Control (no NPs) * LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf) * Time: Incubation time (h) * Date: Date of the measurement * EcoPlate: Number of the EcoPlate * Measurement: Measurement number (3 measurements per plate) * 32 substrates (final 32 columns): OD at 590 nm measured for the given substrate PIXE_(...) PIXE measurements (Fig. 1/ Table S2): * ExposureConcentration: Nominal concentration of nTiO2 (mg/L) * Replicate: Replicate number * EggCount: Number of eggs that was analyzed * Date: Date at which the sample was taken * mL_EggWater: Volume of egg water (mL) that was analyzed along with the eggs * Element: Measured element * Total_ppm: Elemental concentration (ppm) * LOD_ppm: LOD (ppm) * ng_Element_per_egg: Elemental concentration (ng/egg) * LOD_ng_per_egg: LOD (ng/egg) TiO2Sedimentation_(...) ICP-OES measurements of Ti for sedimentation analyses (Fig. S4): * Time: Incubation time (h) * ExposureTiO2: Nominal concentration of nTiO2 (mg/L) * MediaTi: Measured concentration of Ti (mg/L) * MediaTiO2: Measured concentration of TiO2 (mg/L) UVspectra_(...) UV-light spectra (Fig. S1): * wavelength: Measured wavelength (nm) * irradiance: Measured irradiance (uW/cm2/nm) * spectrum: Number of the acquired spectrum
本数据集包含Brinkmann等人2021年发表于《水生毒理学》(*Aquatic Toxicology*)的论文《二氧化钛纳米颗粒吸附于斑马鱼卵影响定植菌群》(*Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota*)中的全部实验数据。
16SrRNA_(...):包含格式为FASTA与FASTQ的16S rRNA序列(对应图3c/附表S4),序列描述行包含如下内容:
* 菌落编号,与附表S4中的编号一致;
* 所用测序引物(27F);
* 纳米颗粒暴露组别:纳米二氧化钛(nTiO₂,5 mg/L)或对照组(无纳米颗粒);
* 亲代斑马鱼交配家系编码,用于繁育获得幼体(A7、A8或A9)
2photon_(...):
* 对应图2的双光子扫描图像;
* 图2b/e的感兴趣区域(Region of Interest, ROI)集合(已压缩);
* 波长与聚焦参数文件(.dat格式)
CLSM_(...):共聚焦激光扫描显微镜(Confocal Laser Scanning Microscopy, CLSM)相关数据:
* 对应图3b的CLSM扫描图像(已压缩);
* 感兴趣区域(ROI,已压缩);
* 元数据,其中scanID与压缩数据的扫描编号及ROI编号一一对应
CFUcounts_(...):菌落形成单位(Colony Forming Unit, CFU)计数数据(对应图4/图S7),包含以下字段:
* 暴露化合物:nTiO₂(5 mg/L)、纳米银(nAg,0.25 mg Ag/L)或对照组(无纳米颗粒);
* 发育阶段:分析所用的斑马鱼生命阶段,即胚胎(受精后1天,1 dpf, days post fertilization)或幼体(受精后5天,5 dpf);
* 日期:菌落分离日期;
* 稀释因子:计数时采用的稀释倍数;
* CFU_Larvae:每枚卵或幼体的菌落形成单位数
DLS_(...):动态光散射(Dynamic Light Scattering, DLS)测量数据(对应表2,针对纳米二氧化钛):
* 孵育时间(h);
* 标称浓度(mg/L);
* ZP:Zeta电位(mV);
* d_size:Z平均粒径(nm)
DoseResponses_(...):3至5 dpf期间暴露于nTiO₂的斑马鱼幼体死亡率数据(对应图S6),包含以下字段:
* 标称暴露浓度(mg/L);
* 暴露时长(天);
* 死亡率:死亡幼体所占百分比;
* 评分日期:死亡率统计日期
EcoPlate_(...):EcoPlate测量数据(对应图5/图S3/图S8/图S9),包含以下字段:
* 暴露化合物:斑马鱼卵暴露组别:nTiO₂(5 mg/L)、nAg(0.25 mg Ag/L)或对照组(无纳米颗粒);
* 发育阶段:分析所用的斑马鱼生命阶段,即胚胎(1 dpf)或幼体(5 dpf);
* 孵育时间(h);
* 测量日期;
* EcoPlate编号;
* 测量次数:每块板的3次重复测量结果;
* 32种底物(共32列数据):各底物在590 nm处的光密度(Optical Density, OD)值
PIXE_(...):质子诱导X射线发射(Particle-Induced X-ray Emission, PIXE)测量数据(对应图1/附表S2),包含以下字段:
* 暴露浓度:nTiO₂的标称浓度(mg/L);
* 重复次数;
* 分析卵的数量;
* 采样日期;
* 卵水体积(mL):与卵一同分析的卵水体积;
* 被测元素;
* 元素浓度(ppm);
* 检测限(Limit of Detection, LOD,ppm);
* 每枚卵的元素含量(ng/egg);
* 每枚卵的检测限(LOD,ng/egg)
TiO2Sedimentation_(...):用于沉降分析的钛元素电感耦合等离子体光学发射光谱(Inductively Coupled Plasma-Optical Emission Spectrometry, ICP-OES)测量数据(对应图S4),包含以下字段:
* 孵育时间(h);
* nTiO₂的标称浓度(mg/L);
* 介质中钛的实测浓度(mg/L);
* 介质中二氧化钛的实测浓度(mg/L)
UVspectra_(...):紫外光谱数据(对应图S1),包含以下字段:
* 测量波长(nm);
* 辐照度(μW/cm²/nm);
* 采集的光谱编号
创建时间:
2024-01-23



